Antiproliferative and cytotoxic effects of metformin were tested in human sknbe2 and sh-sy5y neuroblastoma cell lines and in sknbe2 cells in which differentiation is induced by retinoic acid treatment or stable overexpression of ndm29 non-coding rna, both conditions characterized by a neuron-like differentiated phenotype.

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metformin is negligibly bound to plasma proteins and is, therefore, less likely to interact with highly protein-bound drugs such as salicylates, sulfonamides, chloramphenicol, and probenecid as compared to sulfonylureas, which are extensively bound to serum proteins.

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